DRAGEN TSO 500 and TSO 500 ctDNA Analysis Software
  • DRAGEN TSO 500 and TSO 500 ctDNA Analysis Software
  • DRAGEN TSO 500 Guides
    • DRAGEN TSO 500 v2.6
      • Introduction to DRAGEN TSO 500 Analysis Software v2.6.x
      • Getting Started
        • Installation of 2.6.0 on Standalone DRAGEN Server
        • Installation of 2.6.1 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
        • Installation of NovaSeq 6000Dx TSO 500 Analysis Application
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • NovaSeq 6000Dx Run Set Up
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
          • Run on Multiple DRAGEN Servers
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 Analysis on ICA
      • Analysis Output
        • DNA Output
        • RNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
          • RNA Expanded Metrics
          • HRD Metrics Report
        • Coverage Reports
        • Block List
      • Analysis Methods
        • FastQ Generation
        • DNA Analysis Methods
        • RNA Analysis Methods
        • Quality Control
        • Performance Testing
      • Troubleshooting
    • DRAGEN TSO 500 v2.5
      • Introduction to DRAGEN TSO 500 Analysis Software v2.5.x
      • Getting Started
        • Installation of 2.5.3 on Standalone DRAGEN Server
        • Installation of v2.5.4 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
          • Run on Multiple DRAGEN Servers
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 Analysis on ICA
      • Analysis Output
        • DNA Output
        • RNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
          • RNA Expanded Metrics
          • HRD Metrics Report
          • Block List
        • Analysis Methods
          • FASTQ Generation
          • DNA Analysis Methods
          • RNA Analysis Methods
          • Quality Control
          • Performance Testing
      • Troubleshooting
  • DRAGEN TSO 500 ctDNA Guides
    • DRAGEN TSO 500 ctDNA v2.6
      • Introduction to DRAGEN TSO 500 ctDNA Analysis Software v2.6
      • Getting Started
        • Installation of TSO 500 ctDNA v2.6.0 on Standalone DRAGEN Server
        • Installation of TSO 500 ctDNA v2.6.2 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
        • Installation of NovaSeq 6000Dx TSO 500 ctDNA Analysis Application
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • NovaSeq 6000Dx Run Set Up
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 ctDNA Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 ctDNA Analysis on ICA
      • Analysis Output
        • DNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
        • Coverage Reports
        • Block List
      • Analysis Methods
        • FastQ Generation
        • DNA Analysis Methods
        • Quality Control
      • Troubleshooting
  • Reference
    • Revision History
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  • Installation Instructions
  • Running the System Check
  • Uninstall Software

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  1. DRAGEN TSO 500 ctDNA Guides
  2. DRAGEN TSO 500 ctDNA v2.6
  3. Getting Started

Installation of TSO 500 ctDNA v2.6.0 on Standalone DRAGEN Server

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Last updated 4 months ago

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When installing DRAGEN TruSight Oncology 500 and DRAGEN TruSight Oncology 500 ctDNA software on the same DRAGEN server, install the software with the highest corresponding DRAGEN Software version last, as versions below v2.6.0 will overwrite with its corresponding DRAGEN Software version.

If a prior version of DRAGEN TruSight Oncology 500 ctDNA Analysis Software (eg. v2.5.0) is installed after v2.6.0, re-execute the installation script for v2.6.0 to install the compatible version of DRAGEN Software without impacting other installations.

Installation Instructions

As a root user, perform the following steps to install DRAGEN TruSight Oncology 500 ctDNA v2.6.0 Analysis Software:

  1. Contact Illumina Customer Care at to obtain the DRAGEN TruSight Oncology 500 Analysis Software installer package.

  2. Download the installation package provided in the email from Illumina. The link expires after 7 days.

It is recommended to use a command line tool like wget or curl to download the file rather than pasting the link into the web browser bar. For example:

curl -o {filename} "{link}"

wget -O {filename} "{link}"

Where the file name is the installation script file name, and the link is provided by Illumina Customer Care.

  1. Make sure no other analysis is being performed. Installing the software while performing other analyses prevent the installer process from proceeding

  2. Copy the install script to the /staging directory to store the script in the directory.

Installation Script: install_DRAGEN_TSO500_ctDNA-2.6.0.run

MD5sum values v2.6.0: sha256:1ce33d63c1a499768af7e238d41f81c29dea3e5fe5cc2b08abf12b8afec5bd89 v2.6.1: sha256:bbd07dc63a9da517414460218dd9c12fde88a7378178c214ed2b8beba0f9483f

  1. Use the following command to update the run script permission: chmod +x /staging/install_DRAGEN_TSO500_CTDNA-2.6.0.run

  2. Use the following command to run the installation script, which runs for approximately 20 minutes:

    1. For Docker, use the following command: sudo TMPDIR=/staging /staging/install_DRAGEN_TSO500_CTDNA-2.6.0.run . The script installs compatible DRAGEN software and removes any previously installed versions.

    2. For Apptainer, use the following command: sudo TMPDIR=/staging /staging/install_DRAGEN_TSO500_CTDNA-2.6.0.run -- --noDockerInstall This will not install Apptainer, but will install the analysis software in the Singularity Image File (SIF) format and modify the software to launch analyses using Apptainer.

  3. During the installation process, you might be instructed to reboot or power cycle the system to complete the installation of the DRAGEN software. A power cycle of the system requires the server be shut down and restarted.

  4. Log out of the server and then log back in.

  5. Install your DRAGEN server licenses if needed:

    1. To run DRAGEN TruSight Oncology 500 ctDNA v2.6.0 Analysis Software, you need TSOCombined license. This license is pre-installed on DRAGEN servers purchased after August 2022. To check if the license is already installed, run /opt/edico/bin/dragen_liccommand.

    2. For servers connected to the Internet, install your software licenses as follows:

      1. First, test and confirm that the server is connected to the Internet. Example: ping www.illumina.com

      2. To install the license, enter: /opt/edico/bin/dragen_lic -i auto

    3. For servers not connected to the internet, contact Illumina Customer Care at for license information.

  6. After installing DRAGEN server licenses, generate a list of installed DRAGEN server licenses by running the following command: /opt/edico/bin/dragen_lic

    If license installation is successful, the list should include TSOCombined.

    If the expected licenses are not installed, contact Illumina Customer Care.

Running the System Check

After installation is complete, make sure the system functions properly by running the following command: /usr/local/bin/check_DRAGEN_TSO500_CTDNA-2.6.0.sh

The script checks that:

  • All required services are running

  • Proper Docker image is installed

  • DRAGEN TruSight Oncology 500 ctDNA Analysis Software can successfully process a test data set

The system check script runs for approximately 25 minutes. If the script prints a failure message, contact Illumina Technical Support and provide the /staging/check_DRAGEN_TSO500_CTDNA_<timestamp>.tgz output file.

If using MacOS to connect to a server, an error can occur if the local settings are not in English. To resolve the error, disable the ability to set environment variables automatically in Terminal settings.

Uninstall Software

The DRAGEN TruSight Oncology 500 Analysis Software installation includes an uninstall script called uninstall_DRAGEN_TSO500_CTDNA-2.6.0.sh, which is located in /usr/local/bin.

Executing the uninstall script removes the following assets:

  • All DRAGEN TruSight Oncology 500 ctDNA Analysis Software related scripts located in /usr/local/bin

  • Resources found in /staging/illumina/DRAGEN_TSO500_CTDNA

  • The dragen_tso500_ctdna Docker image

To uninstall the DRAGEN TruSight Oncology 500 ctDNA Analysis Software, run the following command as a root user:

uninstall_DRAGEN_TSO500_CTDNA-2.6.0.sh

You are not required to uninstall Docker or DRAGEN software. To remove Docker, review the install instructions for your operating system in the Docker documentation.

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customercare@illumina.com