DRAGEN TSO 500 and TSO 500 ctDNA Analysis Software
  • DRAGEN TSO 500 and TSO 500 ctDNA Analysis Software
  • DRAGEN TSO 500 Guides
    • DRAGEN TSO 500 v2.6
      • Introduction to DRAGEN TSO 500 Analysis Software v2.6.x
      • Getting Started
        • Installation of 2.6.0 on Standalone DRAGEN Server
        • Installation of 2.6.1 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
        • Installation of NovaSeq 6000Dx TSO 500 Analysis Application
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • NovaSeq 6000Dx Run Set Up
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
          • Run on Multiple DRAGEN Servers
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 Analysis on ICA
      • Analysis Output
        • DNA Output
        • RNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
          • RNA Expanded Metrics
          • HRD Metrics Report
        • Coverage Reports
        • Block List
      • Analysis Methods
        • FastQ Generation
        • DNA Analysis Methods
        • RNA Analysis Methods
        • Quality Control
        • Performance Testing
      • Troubleshooting
    • DRAGEN TSO 500 v2.5
      • Introduction to DRAGEN TSO 500 Analysis Software v2.5.x
      • Getting Started
        • Installation of 2.5.3 on Standalone DRAGEN Server
        • Installation of v2.5.4 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
          • Run on Multiple DRAGEN Servers
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 Analysis on ICA
      • Analysis Output
        • DNA Output
        • RNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
          • RNA Expanded Metrics
          • HRD Metrics Report
          • Block List
        • Analysis Methods
          • FASTQ Generation
          • DNA Analysis Methods
          • RNA Analysis Methods
          • Quality Control
          • Performance Testing
      • Troubleshooting
  • DRAGEN TSO 500 ctDNA Guides
    • DRAGEN TSO 500 ctDNA v2.6
      • Introduction to DRAGEN TSO 500 ctDNA Analysis Software v2.6
      • Getting Started
        • Installation of TSO 500 ctDNA v2.6.0 on Standalone DRAGEN Server
        • Installation of TSO 500 ctDNA v2.6.2 on Standalone DRAGEN Server
        • Getting Started on Illumina Connected Analytics
        • Installation of NovaSeq 6000Dx TSO 500 ctDNA Analysis Application
      • Run Planning
        • Sample Sheet Introduction
        • Sample Sheet Requirements
        • Sample Sheet Creation in BaseSpace Run Planning tool
        • NovaSeq 6000Dx Run Set Up
        • Sample Sheet Templates
      • Launching Analysis
        • Analysis Launch on Standalone DRAGEN Server
          • Command-Line Options
        • Analysis Launch on ICA
          • Auto-Launch of DRAGEN TSO 500 ctDNA Analysis on ICA
          • Manual Launch of DRAGEN TSO 500 ctDNA Analysis on ICA
      • Analysis Output
        • DNA Output
        • Combined Variant Output
        • Metrics Output
          • DNA Expanded Metrics
        • Coverage Reports
        • Block List
      • Analysis Methods
        • FastQ Generation
        • DNA Analysis Methods
        • Quality Control
      • Troubleshooting
  • Reference
    • Revision History
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  • Sample Data
  • Sample Settings

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  1. DRAGEN TSO 500 ctDNA Guides
  2. DRAGEN TSO 500 ctDNA v2.6
  3. Run Planning

NovaSeq 6000Dx Run Set Up

The following instructions describe steps to set up a run on NovaSeq 6000Dx Analysis Application.

Use the following steps to configure a TruSight™ Oncology 500 ctDNA run in Illumina Run Manager.

  1. Go to the "Runs" section of Illumina Run Manager by selecting "Runs" on the left-hand side.

  2. Enter sample data manually or by importing a sample sheet

  3. To enter sample data run manually, select “Create Run”.

  4. Choose "DRAGEN TruSight™ Oncology 500 ctDNA Analysis Application" from the "Create Run" screen to set-up and analyze runs for TruSight Oncology 500 ctDNA assay.

Run Settings

  1. On the "Run Settings" screen, enter a run name with the following criteria:

    1. 1 - 40 characters.

    2. Alphanumeric characters, underscores, or dashes only.

    3. Unique across all runs on the instrument.

    The run name identifies the run from sequencing through analysis.

  2. [Optional] Enter a run description. The run description must have the following criteria:

    1. 1 - 50 characters.

    2. Alphanumeric characters or spaces only.

    3. Spaces must be preceded and followed by an alphanumeric character.

  3. Select kit used during library preparation:

    1. TruSight Oncology 500 ctDNA

    2. TruSight Oncology 500 ctDNA v2

  4. Index adapter kit will be automatically selected based on the library prep kit selection

  5. [Optional] Enter a library tube ID.

Depending on the library prep kit selected, additional fields will be populated for run settings and are not editable. Read and index lengths will differ between library prep kit type.

Sample Data

Use the table on the "Sample Data" screen to enter sample information manually.

  1. Select lane information. Options include one to four, or all lanes.

  2. Enter a unique sample ID in the sample ID field with the following criteria:

    1. Controls should be added first.

    2. 1 - 40 characters.

    3. Alphanumeric characters, underscores, or dashes only.

    4. Underscores and dashes must be preceded and followed by an alphanumeric character.

  3. Select an index set ID for the DNA library prepared from the sample.

  4. [Optional] Enter a library name.

Depending on the options selected for index set ID, additional fields will be auto-populated for sample data and are not editable.

Sample Settings

Use the table on the "Sample Settings" screen to enter additional sample information.

  1. [Optional] Enter a sample name with the following criteria:

    1. 1 - 50 characters.

    2. Alphanumeric characters, dashes, underscores, or spaces.

    3. Spaces, underscores, and dashes must be preceded and followed by an alphanumeric character.

  2. [Optional] Enter a sample description with the following criteria:

    1. 1 - 50 characters.

    2. Alphanumeric characters, dashes, underscores, or spaces.

    3. Spaces, underscores, and dashes must be preceded and followed by an alphanumeric character.

Additional fields will be auto-populated based on selections made in the Sample Data screen, which are not editable.

Before starting your run, review that the information entered is correct in the “Run Review” page before saving.

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Last updated 4 months ago

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Alternately, select Import Samples to upload sample information. Refer to for sample sheet requirements.

NovaSeq 6000Dx Analysis Application: Sample Sheet Requirements