# Sample Sheet Templates

DRAGEN TSO 500 ctDNA analysis software is compatible with several instruments and assay workflows, each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

{% hint style="info" %}
For interactive run planning or to create a sample sheet for ICA Autolaunch, use [BaseSpace Run Planner](https://basespace.illumina.com/) to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to [Sample Sheet Creation in BaseSpace Run Planner](https://help.tso500software.illumina.com/dragen-tso-500-ctdna-guides/dragen-tso-500-ctdna-v2.6/run-planning/sample-sheet-creation-in-basespace-run-planning-tool).
{% endhint %}

Users can visit the [Sample Sheet guidelines](https://help.tso500software.illumina.com/dragen-tso-500-ctdna-guides/dragen-tso-500-ctdna-v2.6/run-planning/sample-sheet-requirements) section to learn additional details on required fields and values as they fill in sample information. Use the lookup tables below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration.

{% hint style="success" %}
Note: Templates are not instrument-specific. Users can fill in InstrumentPlatform and InstrumentType according to the [Instrument Lookup](#instrument-lookup) table provided.
{% endhint %}

### v2.6.1-2.6.2

<table data-full-width="true"><thead><tr><th width="190">Assay</th><th width="168.83203125">Instrument</th><th width="160">Assay Workflow</th><th data-type="files">File</th></tr></thead><tbody><tr><td>TSO 500 ctDNA v1</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-4c294424083f6a2ff754ec5a69c39226df22e6f2%2FSampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000-Reverse_Complement-Local.csv?alt=media&#x26;token=41d4ae00-8415-4856-ba71-e1f9fb2487d1">SampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000-Reverse_Complement-Local.csv</a></td></tr><tr><td>TSO 500 ctDNA v1</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-84cba11fdcb49605b758d34fa4229460c8c4c666%2FSampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000Dx-Local.csv?alt=media&#x26;token=4d5af72d-d003-4983-9adb-171005fe3813">SampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000Dx-Local.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-86642c45f370e5d7237f9081936e51bb1777c2c9%2FSampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000-Local.csv?alt=media&#x26;token=2cc21904-e928-4044-a2de-5365b7df0fcb">SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000-Local.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-3eac1f30b65aa17e1ba7330ed22c2938260fad2b%2FSampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000Dx-Local.csv?alt=media&#x26;token=15c77dab-557b-43be-adca-5a8d76e8927f">SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000Dx-Local.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeq X</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-dc115055865e17f1956ebcefeaaa45976fab20c2%2FSampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-NovaseqX-local.csv?alt=media&#x26;token=0324666a-7665-4e37-bb73-3bac006350c0">SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-NovaseqX-local.csv</a></td></tr></tbody></table>

### v2.6.3

<table data-full-width="true"><thead><tr><th width="170.734375">Assay</th><th width="170.15625">Instrument</th><th width="160">Assay Workflow</th><th data-type="files">File</th></tr></thead><tbody><tr><td>TSO 500 ctDNA v1</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-9c9b6410c9fd34a1f122bdc21473932b7c8781ae%2FSampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_rev.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_rev.csv</a></td></tr><tr><td>TSO 500 ctDNA v1</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-e37313744a87151e04446d14462ffed080198fde%2FSampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-1c7f502f925cf94e376c23ea80ac563383d6d231%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_rev.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_rev.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-1783acc33f823ed8d34b206bcadef24698316037%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NovaSeqX</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-1783acc33f823ed8d34b206bcadef24698316037%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2</td><td>NextSeq 2000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-1783acc33f823ed8d34b206bcadef24698316037%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2 w/ v3 indexes</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-f6850175187dc683a58cd45ff863a4b4fdc852b7%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_rev.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_rev.csv</a></td></tr><tr><td>TSO 500 ctDNA v2 w/ v3 indexes</td><td>NovaSeq 6000Dx</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-3219dc74585d2e6a1b4e28b204dd1bca3a178a7d%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2 w/ v3 indexes</td><td>NovaSeq X</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-3219dc74585d2e6a1b4e28b204dd1bca3a178a7d%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv</a></td></tr><tr><td>TSO 500 ctDNA v2 w/ v3 indexes</td><td>NextSeq 2000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-3219dc74585d2e6a1b4e28b204dd1bca3a178a7d%2FSampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv?alt=media">SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv</a></td></tr></tbody></table>

### Instrument Lookup

Use the following InstrumentPlatform values, based on Instrument value, to replace the placeholder value in the above templates:

| Instrument                   | InstrumentPlatform |
| ---------------------------- | ------------------ |
| NovaSeq 6000                 | NovaSeq            |
| NovaSeq 6000Dx (in RUO Mode) | NovaSeq            |
| NovaSeqX                     | NovaSeqXSeries     |
| NextSeq 2000                 | NextSeq1k2k        |