Sample Sheet Templates

Sample Sheet templates for TSO 500 ctDNA standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use BaseSpace Run Planner.

DRAGEN TSO 500 ctDNA analysis software is compatible with several instruments and assay workflows, each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

For interactive run planning or to create a sample sheet for ICA Autolaunch, use BaseSpace Run Planner to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to Sample Sheet Creation in BaseSpace Run Planner.

Users can visit the Sample Sheet guidelines section to learn additional details on required fields and values as they fill in sample information. Use the lookup tables below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration.

Note: Templates are not instrument-specific. Users can fill in InstrumentPlatform and InstrumentType according to the Instrument Lookup table provided.

v2.6.1-2.6.2

Assay	Instrument	Assay Workflow	File
TSO 500 ctDNA v1	NovaSeq 6000	Standard	SampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000-Reverse_Complement-Local.csv
TSO 500 ctDNA v1	NovaSeq 6000Dx (in RUO mode)	Standard	SampleSheet-TSO500_ctDNA-v2_6_1-v1_assay-Novaseq6000Dx-Local.csv
TSO 500 ctDNA v2	NovaSeq 6000	Standard	SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000-Local.csv
TSO 500 ctDNA v2	NovaSeq 6000Dx (in RUO mode)	Standard	SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-Novaseq6000Dx-Local.csv
TSO 500 ctDNA v2	NovaSeq X	Standard	SampleSheet-TSO500_ctDNA-v2_6_1-v2_assay-NovaseqX-local.csv

v2.6.3

Assay	Instrument	Assay Workflow	File
TSO 500 ctDNA v1	NovaSeq 6000	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_rev.csv
TSO 500 ctDNA v1	NovaSeq 6000Dx (in RUO mode)	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v1_assay-i5_fwd.csv
TSO 500 ctDNA v2	NovaSeq 6000	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_rev.csv
TSO 500 ctDNA v2	NovaSeq 6000Dx (in RUO mode)	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv
TSO 500 ctDNA v2	NovaSeqX	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv
TSO 500 ctDNA v2	NextSeq 2000	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-i5_fwd.csv
TSO 500 ctDNA v2 w/ v3 indexes	NovaSeq 6000	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_rev.csv
TSO 500 ctDNA v2 w/ v3 indexes	NovaSeq 6000Dx	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv
TSO 500 ctDNA v2 w/ v3 indexes	NovaSeq X	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv
TSO 500 ctDNA v2 w/ v3 indexes	NextSeq 2000	Standard	SampleSheet-TSO500_ctDNA-v2_6_3-v2_assay-UDv3-i5_fwd.csv

Instrument Lookup

Use the following InstrumentPlatform values, based on Instrument value, to replace the placeholder value in the above templates:

Instrument	InstrumentPlatform
NovaSeq 6000	NovaSeq
NovaSeq 6000Dx (in RUO Mode)	NovaSeq
NovaSeqX	NovaSeqXSeries
NextSeq 2000	NextSeq1k2k