Sample Sheet Requirements

DRAGEN TSO 500 ctDNA Analysis Software has optional and required fields that are required in addition to general sample sheet requirements. Follow the steps below to create a valid samplesheet.

TSO 500L Data Section header changes depending on the deployment:

• Standalone DRAGEN Server and ICA with Manual Launch: TSO500L_Data

• ICA with Auto-launch: Cloud_TSO500L_Data

[TSO500L_Data] Section

Parameter	Required	Details
Sample_ID	Required	The unique ID to identify a sample. The sample ID is included in the output file names. Sample IDs are not case sensitive. Sample IDs must have the following characteristics: - Unique for the run. - 1–40 characters. - No spaces. - Alphanumeric characters with underscores and dashes. If you use an underscore or dash, enter an alphanumeric character before and after the underscore or dash. eg, Sample1-T5B1_022515. - Cannot be called all, default, none, unknown, undetermined, stats, or reports. - Must match a Sample_ID listed in the BCL Convert Data section. - Each sample must have a unique combination of Lane (if applicable), sample ID, and index ID or the analysis will fail.
Sample_Type	Required	Enter DNA
Sample_Description	Not Required	Sample description must meet the following requirements: - 1–50 characters. - Alphanumeric characters with underscores, dashes and spaces. If you enter a underscore, dash, or space, enter an alphanumeric character before and after. eg, Liquid-Sample_213.

To ensure a successful analysis, follow these guidelines:

1. Avoid any blank lines at the end of the sample sheet; these can cause the analysis to fail.

2. When running local analysis using the command line save the sample sheet in the sequencing run folder with the default name SampleSheet.csv, or choose a different name and specify the path in the command-line options.

ICA with Auto-launch: Sample Sheet Requirements

Refer to the following requirements to create sample sheets for running the analysis on ICA with Auto-launch. For sample sheet requirements common between deployments see Standard Sample Sheet Requirements. Samples sheets can be created using BaseSpace Run Planning Tool or manually by downloading and editing a sample sheet template

To auto-launch analysis from the sequencer run folder, ensure the StartsFromFastq and SampleSheetRequested fields are set to FALSE. To auto-launch analysis from FASTQs after BCL Convert auto-launch, StartsFromFastq and SampleSheet Requested fields must be set to TRUE

[Cloud_TSO500L_Data] Section

Refer to [TSO500L_Data] Section for this section's requirements.

[Cloud_TSO500L_Settings] Section

Parameters	Required	Details
SoftwareVersion	Not Required	The TSO500S software version
StartsFromFastq	Required	Set the value to TRUE or FALSE. To auto-launch from BCL files, set to FALSE. To auto-launch from FASTQ files after auto-launch of BCL Convert, set to TRUE.
SampleSheetRequested	Required	Set the value to TRUE or FALSE. To auto-launch from BCL files, set to FALSE. To auto-launch from FASTQ files after auto-launch of BCL Convert, set to TRUE.

[Cloud_Data] Section

Parameter	Required	Details
Sample_ID	Not Required	The same sample ID used in the Cloud_TSO500L_Data section.
ProjectName	Not Required	The BaseSpace project name.
LibraryName	Not Required	Combination of sample ID and index values in the following format: sampleID_Index_Index2
LibraryPrepKitName	Required	The Library Prep Kit used.
IndexAdapterKitName	Not Required	The Index Adapter Kit used.

[Cloud_Settings] Section

Parameter	Required	Details
GeneratedVersion	Not Required	The cloud GSS version used to create the sample sheet. Optional if manually updating a sample sheet.
CloudWorkflow	Not Required	Ica_workflow_1
Cloud_TSO500L_Pipeline	Required	This value is a universal record number (URN) . The valid value is: urn:ilmn:ica:pipeline:850b4fe6-2a2f-4a85-ae57-cf082dfbefd4#DRAGEN_TruSight_Oncology_500_ctDNA_v2_6_1_4
BCLConvert_Pipeline	Required	The value is a URN in the following format: urn:ilmn:ica:pipeline: <pipeline-ID>#<pipeline-name>

NovaSeq 6000Dx Analysis Application: Sample Sheet Requirements

This section describes fields specific for sample sheets for NovaSeq 6000Dx Analysis Application. For more information on DRAGEN TSO 500 ctDNA Analysis Software sample sheet requirements, refer to the sections above.

Mismatches between the samples and index primers can cause incorrect results due to loss of positive sample identification. Enter sample IDs and assign indexes in the sample sheet before beginning library preparation. Record sample IDs, indexes, and plate well orientation for reference during library preparation.

[BCLConvert_Settings] Section

Parameter Name	Required
SoftwareVersion	Required	Enter the IRM iapp software version 2.6.1-4v2