# Sample Sheet Requirements

{% hint style="info" %}
TSO 500L Data Section header changes depending on the deployment:

* Standalone DRAGEN Server and ICA with Manual Launch: `TSO500L_Data`
* ICA with Auto-launch: `Cloud_TSO500L_Data`
  {% endhint %}

### \[TSO500L\_Data] Section

<table><thead><tr><th width="200">Parameter</th><th width="102">Required</th><th>Details</th></tr></thead><tbody><tr><td>Sample_ID</td><td>Required</td><td>The unique ID to identify a sample. The sample ID is included in the output file names. Sample IDs are not case sensitive. Sample IDs must have the following characteristics:<br>- Unique for the run.<br>- 1–40 characters.<br>- No spaces.<br>- Alphanumeric characters with underscores and dashes. If you use an underscore or dash, enter an alphanumeric character before and after the underscore or dash. eg, Sample1-T5B1_022515.<br>- Cannot be called <code>all</code>, <code>default</code>, <code>none</code>, <code>unknown</code>, <code>undetermined</code>, <code>stats</code>, or <code>reports</code>.<br>- Must match a Sample_ID listed in the BCL Convert Data section.<br>- Each sample must have a unique combination of Lane (if applicable), sample ID, and index ID or the analysis will fail.</td></tr><tr><td>Sample_Type</td><td>Required</td><td>Enter <code>DNA</code></td></tr><tr><td>Sample_Description</td><td>Not Required</td><td>Sample description must meet the following requirements:<br>- 1–50 characters.<br>- Alphanumeric characters with underscores, dashes and spaces. If you enter a underscore, dash, or space, enter an alphanumeric character before and after. eg, Liquid-Sample_213.</td></tr></tbody></table>

To ensure a successful analysis, follow these guidelines:

1. Avoid any blank lines at the end of the sample sheet; these can cause the analysis to fail.
2. When running local analysis using the command line save the sample sheet in the sequencing run folder with the default name `SampleSheet.csv`, or choose a different name and specify the path in the command-line options.

## ICA with Auto-launch: Sample Sheet Requirements

Refer to the following requirements to create sample sheets for running the analysis on ICA with Auto-launch. For sample sheet requirements common between deployments see [Standard Sample Sheet Requirements](#standard-sample-sheet-requirements). Samples sheets can be created using BaseSpace Run Planning Tool or manually by downloading and editing a sample sheet template

{% hint style="info" %}
To auto-launch analysis from the sequencer run folder, ensure the StartsFromFastq and SampleSheetRequested fields are set to FALSE. To auto-launch analysis from FASTQs after BCL Convert auto-launch, StartsFromFastq and SampleSheet Requested fields must be set to TRUE
{% endhint %}

### **\[Cloud\_TSO500L\_Data] Section**

Refer to [\[TSO500L\_Data\] Section](#tso500l_data-section) for this section's requirements.

### **\[Cloud\_TSO500L\_Settings] Section**

<table><thead><tr><th width="225">Parameters</th><th width="127">Required</th><th>Details</th></tr></thead><tbody><tr><td>SoftwareVersion</td><td>Not Required</td><td>The TSO500S software version</td></tr><tr><td>StartsFromFastq</td><td>Required</td><td>Set the value to TRUE or FALSE. To auto-launch from BCL files, set to FALSE. To auto-launch from FASTQ files after auto-launch of BCL Convert, set to TRUE.</td></tr><tr><td>SampleSheetRequested</td><td>Required</td><td><p>Set the value to TRUE or FALSE.</p><p>To auto-launch from BCL files, set to FALSE. To auto-launch from FASTQ files after auto-launch of BCL Convert, set to TRUE.</p></td></tr></tbody></table>

### \[Cloud\_Data] Section

<table><thead><tr><th width="228">Parameter</th><th width="139">Required</th><th>Details</th></tr></thead><tbody><tr><td>Sample_ID</td><td>Not Required</td><td>The same sample ID used in the Cloud_TSO500L_Data section.</td></tr><tr><td>ProjectName</td><td>Not Required</td><td>The BaseSpace project name.</td></tr><tr><td>LibraryName</td><td>Not Required</td><td>Combination of sample ID and index values in the following format: sampleID_Index_Index2</td></tr><tr><td>LibraryPrepKitName</td><td>Required</td><td>The Library Prep Kit used.</td></tr><tr><td>IndexAdapterKitName</td><td>Not Required</td><td>The Index Adapter Kit used.</td></tr></tbody></table>

### \[Cloud\_Settings] Section

<table><thead><tr><th width="243">Parameter</th><th width="132">Required</th><th>Details</th></tr></thead><tbody><tr><td>GeneratedVersion</td><td>Not Required</td><td>The cloud GSS version used to create the sample sheet. Optional if manually updating a sample sheet.</td></tr><tr><td>CloudWorkflow</td><td>Not Required</td><td>Ica_workflow_1</td></tr><tr><td>Cloud_TSO500L_Pipeline</td><td>Required</td><td>This value is a universal record number (URN) . The valid value is: urn:ilmn:ica:pipeline:850b4fe6-2a2f-4a85-ae57-cf082dfbefd4#DRAGEN_TruSight_Oncology_500_ctDNA_v2_6_1_4</td></tr><tr><td>BCLConvert_Pipeline</td><td>Required</td><td>The value is a URN in the following format: urn:ilmn:ica:pipeline: &#x3C;pipeline-ID>#&#x3C;pipeline-name></td></tr></tbody></table>

## NovaSeq 6000Dx Analysis Application: Sample Sheet Requirements

This section describes fields specific for sample sheets for NovaSeq 6000Dx Analysis Application. For more information on DRAGEN TSO 500 ctDNA Analysis Software sample sheet requirements, refer to the sections above.

{% hint style="warning" %}
Mismatches between the samples and index primers can cause incorrect results due to loss of positive sample identification. Enter sample IDs and assign indexes in the sample sheet before beginning library preparation. Record sample IDs, indexes, and plate well orientation for reference during library preparation.
{% endhint %}

### \[BCLConvert\_Settings] Section

<table><thead><tr><th width="206">Parameter Name</th><th width="135">Required</th><th></th></tr></thead><tbody><tr><td>SoftwareVersion</td><td>Required</td><td>Enter the IRM iapp software version 2.6.1-4v2</td></tr></tbody></table>