# Sample Sheet Templates

DRAGEN TSO 500 analysis software is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

{% hint style="info" %}
For interactive run planning or to create a sample sheet for ICA Auto-launch, use [BaseSpace Run Planner](https://basespace.illumina.com/) to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to [Sample Sheet Creation in BaseSpace Run Planner](https://help.tso500software.illumina.com/dragen-tso-500-guides/dragen-tso-500-v2.5/run-planning/sample-sheet-creation-in-basespace-run-planning-tool).
{% endhint %}

Users can visit the [Sample Sheet guidelines](https://help.tso500software.illumina.com/dragen-tso-500-guides/dragen-tso-500-v2.5/run-planning/sample-sheet-requirements) section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

<table data-full-width="true"><thead><tr><th width="171">Assay</th><th width="139">Instrument</th><th width="160">Assay Workflow</th><th data-type="files">File</th></tr></thead><tbody><tr><td>TSO500</td><td>NextSeq 550</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-769b47a659ca9d2c463b613fa5d74e7c14e42714%2FTSO%20500%20NextSeq550_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=8c46b87d-e08f-402b-ae36-cbaa7b19cb86">TSO 500 NextSeq550_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NextSeq 550</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-769b47a659ca9d2c463b613fa5d74e7c14e42714%2FTSO%20500%20NextSeq550_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=8c46b87d-e08f-402b-ae36-cbaa7b19cb86">TSO 500 NextSeq550_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-45ced3c038776927e08a68f6a58b220db84c510e%2FTSO%20500%20HRD_NovaSeq6000_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=3d0d6924-c8ac-4243-a0dd-3d50d24b69c8">TSO 500 HRD_NovaSeq6000_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 + HRD</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-f1c727bcc6aa3f2327be17ae2414aadadf3bbb30%2FTSO%20500%20HRD_NovaSeq6000Dx_fwd_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=6cec92eb-ee64-4104-b26b-de8edddda9f7">TSO 500 HRD_NovaSeq6000Dx_fwd_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-2c80bedfd47b0b4972280f35d7674c8aebeb6416%2FTSO%20500%20HT_NovaSeq6000_SP_S1_S2_S4_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=c3a41e54-eed5-4145-b1ed-ea56393a111f">TSO 500 HT_NovaSeq6000_SP_S1_S2_S4_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-594a23db165f059f87294e339f867e6222fbd6c6%2FTSO%20500%20HT_NovaSeq6000_SP_S1_S2_S4_XP_v2%20Sample%20Sheet%20Template.csv?alt=media&#x26;token=7dc3e2cf-7751-4245-a0a4-24515587b080">TSO 500 HT_NovaSeq6000_SP_S1_S2_S4_XP_v2 Sample Sheet Template.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>Standard</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-d78e90af27e47066eb1467f6e5b835532a5d1c75%2FTSO%20500%20HT_NovaSeq6000DxRUO_S1_S2_S4_v2%20Sample%20Sheet%20Template_200037181.csv?alt=media&#x26;token=ee67e1c2-8e78-42a5-b6e6-900cb0fb3c75">TSO 500 HT_NovaSeq6000DxRUO_S1_S2_S4_v2 Sample Sheet Template_200037181.csv</a></td></tr><tr><td>TSO500 HT</td><td>NovaSeq 6000Dx (in RUO mode)</td><td>XP*</td><td><a href="https://3845108255-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2F7XRWgkRPkhoHXVslBqXD%2Fuploads%2Fgit-blob-d00b74429ba9656a2661ec0df156403a70577c59%2FTSO%20500%20HT_NovaSeq6000DxRUO_S1_S2_S4_XP_v2%20Sample%20Sheet%20Template_200037182.csv?alt=media&#x26;token=e66c4e0f-df72-40a6-8f10-f6cebdef234f">TSO 500 HT_NovaSeq6000DxRUO_S1_S2_S4_XP_v2 Sample Sheet Template_200037182.csv</a></td></tr></tbody></table>

\*Lane numbers cannot exceed what is supported by the flow cell in use.