Sample Sheet Templates

Sample Sheet templates for TSO 500 v2.6.0 standalone DRAGEN server and ICA manual launch analysis can be found in the table below. For auto-launch compatible sample sheets, use BaseSpace Run Planner.

DRAGEN TSO 500 analysis software is compatible with several instruments and assay workflows (standard, XP), each of which have implications for the sample sheet.

Sample sheet templates contain all required fields, including index sequences in the proper orientation for all indexes from a given library prep kit. The templates are provided as a starting point for creating a sample sheet manually when launching analysis on a standalone DRAGEN server or on ICA using manual launch.

For interactive run planning or to create a sample sheet for ICA Autolaunch, use BaseSpace Run Planner to create valid sample sheets for either local or cloud analysis. To set up a run in BaseSpace run planner, refer to Sample Sheet Creation in BaseSpace Run Planner.

Users can visit the Sample Sheet guidelines section to learn additional details on required fields and values as they fill-in their sample information. Use the lookup table below to select and download the sample sheet template that matches your instrument, assay, and workflow configuration:

Assay	Instrument	Assay Workflow	File
TSO500	NextSeq 550	Standard	Sample_Sheet_Template_TSO 500_i5Rev_Std_Local.csv
TSO500 + HRD	NextSeq 550	Standard	Sample_Sheet_Template_TSO 500 HRD_i5Rev_Std_Local.csv
TSO500 + HRD	NovaSeq 6000	Standard	Sample_Sheet_Template_TSO 500 HRD_i5Rev_Std_Local.csv
TSO500 + HRD	NovaSeq 6000Dx (in RUO mode)	Standard	Sample_Sheet_Template_TSO 500 HRD_i5Fwd_Std_Local.csv
TSO500 HT	NextSeq 1000 / 2000	Standard	Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv
TSO500 HT	NovaSeq 6000	Standard	Sample_Sheet_Template_TSO 500 HT_i5Rev_Std_Local.csv
TSO500 HT	NovaSeq 6000	XP*	Sample_Sheet_Template_TSO 500 HT_i5Rev_XP_Local.csv
TSO500 HT	NovaSeq 6000Dx (in RUO mode)	Standard	Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv
TSO500 HT	NovaSeq 6000Dx (in RUO mode)	XP*	Sample_Sheet_Template_TSO 500 HT_i5Fwd_XP_Local.csv
TSO500 HT	NovaSeq X	Standard	Sample_Sheet_Template_TSO 500 HT_i5Fwd_Std_Local.csv
TSO500 HT	NovaSeq X	XP*	Sample_Sheet_Template_TSO 500 HT_i5Fwd_XP_Local.csv

*Lane numbers cannot exceed what is supported by the flow cell in use.