*The BaseSpace Sequence Hub setting for run monitoring and storage must be selected on the instrument to use DRAGEN TSO 500 analysis auto-launch. For information on preparing your instrument for DRAGEN TSO 500 auto-launch, refer to the documentation for your instrument.
Use BaseSpace Sequence Hub Run Planning tool or the sample sheet templates provided on the support page to create and export a sample sheet.
If BaseSpace Run Planning tool is not available in your region, use the sample sheet template.
Import the sample sheet to the instrument and start the sequencing run. Refer to for sample sheet guidance.
Data is uploaded to BaseSpace Sequence Hub and then pushed to ICA. You can monitor the run in BaseSpace Sequence Hub.
Analysis auto launches in ICA when sequencing and the upload completes. You can monitor the status of the analysis in BaseSpace Sequence Hub or ICA
View the analysis output results in either BaseSpace Sequence Hub or ICA.
To avoid invalid sample sheet configurations, Illumina recommends using BaseSpace Run Planning tool to generate sample sheets. Using an invalid sample sheet can result in failed runs and analyses.
BaseSpace Sequence Hub Requirements for ICA Auto-Launch
BaseSpace Run Planning tool is a multi-step workflow that generates a manual launch or auto-launch capable sample sheet for export and requires the following additional settings:
Access to BaseSpace Sequence Hub.
ICA Run Storage is enabled under BaseSpace Sequence Hub settings.
Refer to the for information on setting up a BaseSpace Sequence Hub project.
Requeue Analysis
You can requeue analysis of a run via the run's Summary page in BaseSpace Sequence Hub.
Refer to the for more information on requeuing an analysis.
Minimum Storage Requirements on ICA
Sequencing System
Minimum Disk Space (Gb)
Refer to the for information on how to manage accounts and subscriptions.
Guided Examples
Please review these guided examples of using DRAGEN TSO 500 Analysis Software with auto-launch on ICA:
If necessary, you can requeue the analysis via BaseSpace Sequence Hub.
Auto-Launch with FASTQs generated by Standalone BCL Convert Pipeline (Start from FASTQ)
When using BSSH Run Planning to generate a sample sheet to auto-launch analysis on ICA, you must designate "Start from Fastq" to be True or False (default is False).
If you choose "False", BSSH will kick off the TSO 500 pipeline normally using BCL input from the run folder.
If you choose "True" for this option, BSSH will run two ICA pipelines in sequence:
First, it will run the BCL Convert v3.10.9 pipeline to generate the FASTQ files
Second, it will kick off the TSO 500 pipeline using the FASTQ files generated above as the input
Please note. Run QC metrics are generated inside the TSO 500 pipeline from interop files in the run folder. When starting TSO 500 from FASTQ (via auto-launch or manual launch), Run QC metrics will not be generated.
When the auto-launched analysis completed, you will see the analysis result shows results from both the BCL Convert pipeline and the TSO 500 pipeline, like below:
