Sample Sheet Introduction

Overview

A sample sheet is required for each analysis with DRAGEN TruSight Oncology 500 Analysis Software. A sample sheet is a comma-separated value (*.csv) file format used by Illumina instruments, platforms, and analysis pipelines to store settings and data for sequencing and analysis. The DRAGEN TruSight Oncology 500 Analysis Software is compatible with the sample sheet v2. For general information on the sample sheet v2, refer to Illumina Connected Software - Sample Sheet.

The sample sheet includes a list of samples and their index sequences, along with additional information required to run DRAGEN TruSight Oncology 500 Analysis Software. For example, DNA samples with the TruSight Oncology 500 HRD add-on probes must be indicated in the Sample Feature column of the sample sheet. Appropriate index adapter sequences are determined by the assay used to perform analysis.

When running analysis on a standalone DRAGEN server or on ICA, a valid sample sheet can be created by:

• BaseSpace Run Planner (preferred), see Sample Sheet Creation in BaseSpace Run Planner page for details

• Downloading and modifying a sample sheet template following the requirements, see Sample Sheet Requirements page for details

When running analysis using a NovaSeq 6000Dx Analysis Application, a valid sample sheet can be created by:

• Using the user interface of the DRAGEN TruSight Oncology 500 Analysis Application, see Run Planning on Illumina Run Manager for details

• Downloading and modifying a sample sheet template following the requirements (see Sample Sheet Requirements page for details), then importing it to Illumina Run Manager.

The run set up section of this guide includes specific instructions to plan a run and set up a valid sample sheet for each deployment of DRAGEN TruSight Oncology 500 Analysis Software.