Installation of NovaSeq 6000Dx TSO 500 Analysis Application

Instructions to install DRAGEN TSO 500 Analysis Application on NovaSeq 6000Dx (RUO mode).

Illumina distributes Illumina DRAGEN TruSight Oncology 500 (HRD) Analysis Application on NovaSeq 6000Dx globally (except Japan) for TSO 500 assay with and without HRD add-on kit. In Japan, Illumina distributes Illumina DRAGEN TruSight Oncology 500 Analysis Application on NovaSeq 6000Dx for TSO 500 assay without HRD.

Prerequisites

• A NovaSeq 6000Dx sequencing instrument with paired DRAGEN server v4.

• Illumina Run Manager installed by Illumina support personnel.

• HRD license installed on the DRAGEN server v4 (not required for customers in Japan)

• The user installing the app must have admin privileges on Illumina Run Manager.

Installation Instructions:

1. Contact Illumina Customer Care at [e-mail] to obtain installation package for Illumina DRAGEN TruSight Oncology 500 (HRD) Analysis Application on NovaSeq 6000Dx

   1. If you are a customer in Japan, request Illumina DRAGEN TruSight Oncology 500 Analysis Application on NovaSeq 6000Dx

2. Download the installation package provided in the email by Illumina Customer Care. The link will expire after 7 days.

It is recommended to use a command line tool like wget or curl to download the file rather than pasting the link into the web browser bar. For example:

curl -o {filename} "{link}"

wget -O {filename} '{link}'

Where the file name is the name of either DRAGEN TSO 500 Analysis Application file or DRAGEN ires file, and the link is provided by Illumina Customer Care.

3. The installation package contains the following:

   1. DRAGEN TSO 500 Analysis Application: DRAGEN_TSO500HRD_v2.6.0-2v12.iapp

      1. If you are a customer in Japan, you will be provided DRAGEN_TSO500S_v2.6.0-2v12.iapp

   2. DRAGEN ires: drageninstaller_3.10.17-8.el8.x86_64_prod.ires

MD5sum for the installation package contents are as follows:

• drageninstaller_3.10.17-8.el8.x86_64_prod.ires

  • 12df06502776d8b673c73fb714dc466a

• DRAGEN_TSO500HRD_v2.6.0-2v12.iapp

  • dc4fe5fe2fc3eca57969e978886dcedf

• DRAGEN_TSO500S_v2.6.0-2v12.iapp

  • 74c11da34aeac8fba616bc6763cededa

4. Install the DRAGEN version using the ires:

   1. Log into Illumina Run Manager as a user with admin credentials

   2. Navigate to the top left menu to "DRAGEN" in the drop down

   3. Select "Add DRAGEN Installer" and upload the DRAGEN ires file

   4. The installation is complete once the DRAGEN version 3.10.17 is in the install version(s) list.

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