> For the complete documentation index, see [llms.txt](https://help.tso500software.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.tso500software.illumina.com/dragen-tso-500-guides/mtap-spike-in-resources.md).

# Running DRAGEN Enrichment for supplementary analysis

## Overview and Prerequisites

{% hint style="warning" %}
**Important:** This how-to describes a supplemental research workflow for libraries prepared with spike-in probes added to TSO 500 enrichment (for example, *MTAP* probe spike-in). DRAGEN Enrichment can be used alongside standard TSO 500 analysis to call variants in spike-in target regions.

This workflow extends the standard TSO 500 pipeline and is intended for research use.
{% endhint %}

The TSO 500 HRD pipeline is built for the core TSO 500 panel with the option for HRD probe inclusion. To analyze additional target regions captured with spike-in probes, separately run DRAGEN Enrichment on the same sequencing data.

Follow this high-level workflow and choose a data analysis option under Step 3.

1. Prepare libraries using spike-in protocol (wet lab steps are outside the scope of this software how-to).
2. Sequence the run.
3. Choose a data analysis path:
   1. Run DRAGEN TSO 500 v2.6+ analysis as normal, then use the generated FASTQs to run [DRAGEN Enrichment](/dragen-tso-500-guides/mtap-spike-in-resources/supplemental-cnv-analysis-using-dragen-enrichment.md).
   2. Run [BCL Convert](/dragen-tso-500-guides/mtap-spike-in-resources/bcl-conversion-for-supplementary-analysis.md) first, then use the output FASTQs to run both TSO 500 and [DRAGEN Enrichment](/dragen-tso-500-guides/mtap-spike-in-resources/supplemental-cnv-analysis-using-dragen-enrichment.md) analyses.

Spike-in analysis is currently available for TSO 500 Tissue. This how-to is written for general TSO 500 spike-in workflows; refer to specific add-on workflow pages (e.g., [*MTAP* spike-in](/dragen-tso-500-guides/mtap-spike-in-resources/mtap-add-on-workflow-resources.md)) for necessary resource files and other instructions.

### Current Supplementary Analysis Resources

[MTAP add-on workflow resources](/dragen-tso-500-guides/mtap-spike-in-resources/mtap-add-on-workflow-resources.md)


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