> For the complete documentation index, see [llms.txt](https://help.tso500software.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.tso500software.illumina.com/dragen-tso-500-guides/dragen-tso-500-v2.6/run-planning/sample-sheet-preparation-for-5b-flow-cell.md).

# Sample Sheet Preparation for 5B Flow Cell

{% hint style="info" %}
Data generated by 5B flow cell (NovaSeq X) is only compatible with DRAGEN TSO 500 analysis software v2.6.2
{% endhint %}

NovaSeq X 5B flow cells use a two-step analysis workflow:

1. Generate FASTQ files with BCL Convert v4.4.10.
2. Run variant calling with DRAGEN TSO 500 v2.6.2 from FASTQ files.

To create a sample sheet to enable 5B data analysis, follow the steps below.

{% stepper %}
{% step %}

### Create and export the sample sheet

1. Follow [Sample Sheet Creation in BaseSpace Run Planning tool](/dragen-tso-500-ctdna-guides/dragen-tso-500-ctdna-v2.6/run-planning/sample-sheet-creation-in-basespace-run-planning-tool.md).
2. Set **Starts from Fastq** to `True`.
3. Export the generated sample sheet.
   {% endstep %}

{% step %}

### Update the BCL Convert pipeline URN

1. Open the exported file in a text editor.
2. Use **Find and Replace** to replace this value:

```
urn:ilmn:ica:pipeline:a0778b16-f318-40df-ae04-95998e3a7564#BCL_Convert_v3_10_9_for_TSO500
```

with this value:

```
urn:ilmn:ica:pipeline:4bdae602-abe4-4230-b0c6-f886af563b26#BclConvert_v4_4_10
```

3. Save the modified sample sheet.
   {% endstep %}

{% step %}

### Use the updated file

Upload the updated sample sheet to the pre-planned sequencing run on NovaSeq X.
{% endstep %}
{% endstepper %}


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