Input Checks
Items to check before submitting your analysis
Input Validation Steps
In addition to sample sheet validation, before starting an analysis, the software performs the following checks:
Resource bundle integrity
DRAGEN license validity
NEW in v2.6.2! Instrument Type determination
Instrument type is required by the software because different instrument platforms produce output with distinct systematic noise profiles. DRAGEN TSO 500 analysis software requires instrument-specific baseline noise files. Before kicking off analysis, the software determines the instrument type in order to select which baseline files to use. This means that even when starting from FASTQ, all samples in the run must be sequenced on the same type of instrument.
Instrument Type Determination for Baselines
When starting analysis from BCL files, the software references the RunInfo.xml
file in the Run folder to identify the sequencing instrument type. For analyses beginning with FASTQ files, the software reads the FASTQ file headers for this information. Correct baselines for variant calling are then applied based on the determined instrument type.
For analysis starting from FASTQ files, if a mixed instrument type is detected, the software will exit with an error.
The Combined Variant Output File will include the instrument type Combined Variant Output
Baselines are selected based on a combination of Library Prep Kit and Instrument Type.
Library Prep Kit: TSO500_v2
Instrument Types:
NovaSeq 6000, NovaSeq 6000Dx, NextSeq 500, NextSeq 550: Use one set of baselines.
NextSeq 1000/ 2000: Use a different set of baselines.
NovaSeqX, NovaSeqXPlus: Use another set of baselines.
Library Prep Kit: TSO500 or TSO500HT
A single set of baselines applies to all instrument types.
If instrument type is undetermined (ie not matching any of the below), the software will set instrument type to NA, and the baselines for NovaSeq 6000, NovaSeq 6000Dx, NextSeq 500, and NextSeq 550 will be applied.
A
NovaSeq6000
ADX
NovaSeq6000Dx
NS50 or NB50 or NL50
NextSeq500
NS55 or NB55 or NDX
NextSeq550
VH
NextSeq1k2k
LH
NovaSeqXPlus
LL
NovaSeqX
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